On the Phosphotungstates of Certain Aminoacids

نویسندگان

  • P. A. Levene
  • W. A. Beatty
چکیده

In a communication to the Chemical Society ~ one of us reported on the property of aminoacids to form insoluble phosphotungstates. In a later publication 2 the writers showed the degree of precipitability of aminoacids by means of phosphottmgstic acid, and stated that a separation of aminoacids may be accomplished by means of this reagent. The at tempt was made to apply the method to the study of the acids resulting from the hydrolysis of gelatine by means of mineral acids. Unfortunately, through an accident in the laboratory, the greater part of the material for this study was lost, and hence in this place there will be reported a method of the separation of oxyprolin and of an acid having the composition CTH14N202. Oxyprolin.--This communication is prompted partly by a statement made by Emil Fischer in a recent address delivered to the German Chemical Society in Berlin. 3 Fischer points out that considerable difficulty is experienced in obtaining oxyprolin from the mixture of. the products resulting from hydrolysis of proteids. The process employed by Fischer consisted in removing most of the aminoacids first by esterification, then by crystallization, and finally by means of phosphotungstic acid. From the mother liquid, remaining after all these precipitations, oxyprolin was separated. We have observed that oxyprolin can be isolated as a phosphotungstate without great difficulty. I t precipitates in the form of an oil which crystallizes on standing and is soluble in an excess of the reagent. Its separation was accomplished in the following manner:

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عنوان ژورنال:
  • The Journal of Experimental Medicine

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2003